Fig. 5.

Polyphyllin I treatment downregulated the expressions of Notch signaling pathway-related genes in HCT8 cells. A. After treating HCT8 cells with 5 μM PPI for 24 h, mRNA expression of the Notch signaling receptor Notch1 was detected. B. After treating HCT8 cells with 5 μM PPI for 24 h, mRNA expression of the Notch signaling ligand Jagged1 was detected. C. After treating HCT8 cells with 5 μM PPI for 24 h, mRNA expression of the Notch signaling downstream target gene Hes1 was detected. Data were presented as the means ± SD, n = 3, *P < 0.05, **P < 0.01, ***P < 0.001 vs Control.