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. 2024 Aug 20;27(9):110773. doi: 10.1016/j.isci.2024.110773

Figure 2.

Figure 2

Periodic fasting and time-restricted feeding ameliorate and reverse polycystic kidney disease progression in adult Cy/+ Rats

(A) Schematic of PF and TRF feeding experimental timeline and time-restricted feeding schedule for adult Cy/+ rats.

(B) Hematoxylin and Eosin stained of 12-week-old male wild-type ad libitum and male polycystic ad libitum, PF and TRF rats. Scale = 5mm (Top); 100μm (Bottom).

(C) 2-kidney over body weight of ad libitum, PF and TRF male wild-type and polycystic rats.

(D) Serum creatinine of ad libitum, PF and TRF male wild-type and polycystic rats.

(E) Change in the cystic area between 8-week-old ad libitum and 12-week-old ad libitum, PF and TRF male polycystic rats.

(F) Cyst number per mm2 of kidney from ad libitum, PF and TRF male polycystic rats.

(G) Weekly average animal masses of ad libitum, PF and TRF male wild-type and polycystic rats.

(H) Weekly blood BHB and blood glucose averages from male wild-type and polycystic rats. (Left) Blood measurements were taken before fasting (time 0) and at the end of each 2-day fast for periodic fasted rats. (Right) The weekly blood values for time-restricted rats collected at the end of the time-restricted fasting period.

(I) Immunofluorescence of phospho-S6235/236 and the change in cytoplasmic phospho-S6235/236 signals within cystic epithelia between 8-week-old ad libitum and 12-week-old ad libitum, PF and TRF male polycystic rats. Scale = 50μm.

(J) Sirius Red and Fast Green stain for collagen (red) and the change in collagen deposition between 8-week-old ad libitum and 12-week-old ad libitum, PF and TRF male polycystic rats.

(K) Immunofluorescence of Smooth Muscle Actin (SMA-1) and the change in SMA-1 signal between 8-week-old ad libitum and 12-week-old ad libitum, PF and TRF male polycystic rats. Scale = 50μm Scale = 70μm.

(L) Immunofluorescence of Ki67 and the quantification of Ki67 signal in cysts/tubules and interstitial cells from ad libitum, PF and TRF adult male polycystic rats. Scale = 50μm.

(See also Figures S3–S6. One-way ANOVA followed by ad hoc Tukey’s test was used for multiple comparisons. Mean and standard deviations are shown. ∗ = p < 0.05, ∗∗ = p < 0.01, ∗∗∗ = p < 0.001, ψ = p < 0.0001).