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. 2024 Aug 20;27(9):110773. doi: 10.1016/j.isci.2024.110773
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Rabbit α-Smooth Muscle Actin Abcam Cat# ab5694; RRID:AB_2223021
Rabbit α-Nrf2 Thermo Fisher Scientific Cat# PA5-27882; RRID:AB_2545358
Rabbit α-PGC1a Novus Cat# NBP1-04676; RRID:AB_1522118
Rabbit α-BDH1 Novus Cat# NBP1-88673; RRID:AB_11009202
Rabbit α-OXCT1 Sigma-Aldrich Cat# HPA012047; RRID:AB_1854857
Mouse α-CPT1α Abcam Cat# ab128568; RRID:AB_11141632
Mouse α-Actin Sigma-Aldrich Cat# A5441; RRID:AB_476744
Mouse α-Ki67, Clone B56 (RUO) BD Biosciences Cat# 550609; RRID:AB_393778
Rabbit α-phospho-S6 Ribosomal Protein (Ser235/236) Cell Signaling Technology Cat# 2211; RRID:AB_331679
Rabbit α-phospho-GSK-3β (Ser9) Cell Signaling Technology Cat# 9336; RRID:AB_331405
Rabbit α-Total-GSK-3β (DgC5Z) XP® Cell Signaling Technology Cat# 12456; RRID:AB_2636978
Goat α-rabbit HRP Jackson ImmunoResearch Labs Cat# 111-035-144; RRID:AB_2307391
Goat α-mouse HRP Jackson ImmunoResearch Labs Cat# 115-035-044; RRID:AB_2338503
Goat α-rabbit AlexFluor 594 Thermo Fisher Scientific Cat# A-11012; RRID:AB_2534079
Goat α-mouse AlexaFluor 594 Thermo Fisher Scientific Cat# A-11005; RRID:AB_2534073
Rabbit α-Podocin Thermo Fisher Scientific Cat# PA5-79757; RRID:AB_2746872

Chemicals, peptides, and recombinant proteins

Sodium D-BHB and L-BHB Shaoxing King-Year Biochemical Co., Ltd CAS ID: 300-85-6
PicoLab Rodent Diet 20 Lab Diet Cat#5053
D/L-β-hydroxybutyrate, KetoForce Ketosports
Zymo-spin TM IICR column mini-prep Zymo Research Cat# D4068
Sirius Red/Fast Green collagen staining kit Chondrex Cat# 9046
IGF-1 Rat ELISA Kit Invitrogen Cat# ERIGF1
D-beta-hydroxybutyric acid (D-BHB) Sigma Aldrich CAS:625-72-9; Cat# 54920
L-beta-hydroxybutyric acid (L-BHB) Sigma Aldrich CAS:6168-83-8; Cat# 54925
Sodium d4-DL-beta-hydroxybutyrate (Na d4-DL-BHB) Cayman Chemical CAS:1219804-68-8; Cat# 14158
S-1-(2-pyrrolidinylmethyl)-pyrrolidine (S-PMP) Sigma Aldrich CAS:51207-66-0; Cat# P12411G
triphenylphosphine (TPP) Sigma Aldrich CAS:603-35-0; Cat# 140420250
2,2’-dipyridyl disulfide (DPDS) Sigma Aldrich CAS:2127-03-9; Cat# D11145G
LCMS Grade Water with 0.1% Formic Acid (v/v) Fisher Scientific CAS:7732-18-5; 64-18-6; Cat# LS118-4
LCMS Grade Acetonitrile with 0.1% Formic Acid (v/v) Fisher Scientific CAS:75-05-8; 64-18-6; Cat# LS120-4
LCMS Grade Acetonitrile Fisher Scientific CAS:75-05-8; Cat# A995-4
LCMS Grade Methanol Fisher Scientific CAS:67-56-1; Cat# A456-4
MilliQ Water Fisher Scientific CAS:7732-18-5
Phosphatase inhibitor cocktail 2 & 3 Sigma-Aldrich Cat# P5726; Cat# P0044
pepstatin E110 Chemicon CAS:26305-03-3
leupeptin E18 Chemicon CAS:103476-89-7
antipain E13 Chemicon CAS:149116-08-5
Benzamidine Sigma-Aldrich CAS:618-39-3
Traysol Bayer CAS:9087-70-1

Critical commercial assays

QuantiChrom creatine assay kit BioAssay Systems Cat# DICT-500
Precision Xtra Abbott Cat# 9881465
Contour Next EZ Bayer Cat# 193725201

Experimental models: Organisms/strains

"Ksp-Cre" Mice, B6.Cg-Tg(Cdh16-cre)91Igr/J Jackson Laboratory JAX:012237; MGI:4412080
Pkd1RC/RC Mice, p.Arg3277Cys University of Augusta, Augusta, Georgia
Han Rat, Anks6PKD RRID:RGD_11535000