Antibodies |
|
Rabbit α-Smooth Muscle Actin |
Abcam |
Cat# ab5694; RRID:AB_2223021
|
Rabbit α-Nrf2 |
Thermo Fisher Scientific |
Cat# PA5-27882; RRID:AB_2545358
|
Rabbit α-PGC1a |
Novus |
Cat# NBP1-04676; RRID:AB_1522118
|
Rabbit α-BDH1 |
Novus |
Cat# NBP1-88673; RRID:AB_11009202
|
Rabbit α-OXCT1 |
Sigma-Aldrich |
Cat# HPA012047; RRID:AB_1854857
|
Mouse α-CPT1α |
Abcam |
Cat# ab128568; RRID:AB_11141632
|
Mouse α-Actin |
Sigma-Aldrich |
Cat# A5441; RRID:AB_476744
|
Mouse α-Ki67, Clone B56 (RUO) |
BD Biosciences |
Cat# 550609; RRID:AB_393778
|
Rabbit α-phospho-S6 Ribosomal Protein (Ser235/236) |
Cell Signaling Technology |
Cat# 2211; RRID:AB_331679
|
Rabbit α-phospho-GSK-3β (Ser9) |
Cell Signaling Technology |
Cat# 9336; RRID:AB_331405
|
Rabbit α-Total-GSK-3β (DgC5Z) XP® |
Cell Signaling Technology |
Cat# 12456; RRID:AB_2636978
|
Goat α-rabbit HRP |
Jackson ImmunoResearch Labs |
Cat# 111-035-144; RRID:AB_2307391
|
Goat α-mouse HRP |
Jackson ImmunoResearch Labs |
Cat# 115-035-044; RRID:AB_2338503
|
Goat α-rabbit AlexFluor 594 |
Thermo Fisher Scientific |
Cat# A-11012; RRID:AB_2534079
|
Goat α-mouse AlexaFluor 594 |
Thermo Fisher Scientific |
Cat# A-11005; RRID:AB_2534073
|
Rabbit α-Podocin |
Thermo Fisher Scientific |
Cat# PA5-79757; RRID:AB_2746872
|
|
Chemicals, peptides, and recombinant proteins |
|
Sodium D-BHB and L-BHB |
Shaoxing King-Year Biochemical Co., Ltd |
CAS ID: 300-85-6 |
PicoLab Rodent Diet 20 |
Lab Diet |
Cat#5053 |
D/L-β-hydroxybutyrate, KetoForce |
Ketosports |
|
Zymo-spin TM IICR column mini-prep |
Zymo Research |
Cat# D4068 |
Sirius Red/Fast Green collagen staining kit |
Chondrex |
Cat# 9046 |
IGF-1 Rat ELISA Kit |
Invitrogen |
Cat# ERIGF1 |
D-beta-hydroxybutyric acid (D-BHB) |
Sigma Aldrich |
CAS:625-72-9; Cat# 54920 |
L-beta-hydroxybutyric acid (L-BHB) |
Sigma Aldrich |
CAS:6168-83-8; Cat# 54925 |
Sodium d4-DL-beta-hydroxybutyrate (Na d4-DL-BHB) |
Cayman Chemical |
CAS:1219804-68-8; Cat# 14158 |
S-1-(2-pyrrolidinylmethyl)-pyrrolidine (S-PMP) |
Sigma Aldrich |
CAS:51207-66-0; Cat# P12411G |
triphenylphosphine (TPP) |
Sigma Aldrich |
CAS:603-35-0; Cat# 140420250 |
2,2’-dipyridyl disulfide (DPDS) |
Sigma Aldrich |
CAS:2127-03-9; Cat# D11145G |
LCMS Grade Water with 0.1% Formic Acid (v/v) |
Fisher Scientific |
CAS:7732-18-5; 64-18-6; Cat# LS118-4 |
LCMS Grade Acetonitrile with 0.1% Formic Acid (v/v) |
Fisher Scientific |
CAS:75-05-8; 64-18-6; Cat# LS120-4 |
LCMS Grade Acetonitrile |
Fisher Scientific |
CAS:75-05-8; Cat# A995-4 |
LCMS Grade Methanol |
Fisher Scientific |
CAS:67-56-1; Cat# A456-4 |
MilliQ Water |
Fisher Scientific |
CAS:7732-18-5 |
Phosphatase inhibitor cocktail 2 & 3 |
Sigma-Aldrich |
Cat# P5726; Cat# P0044 |
pepstatin E110 |
Chemicon |
CAS:26305-03-3 |
leupeptin E18 |
Chemicon |
CAS:103476-89-7 |
antipain E13 |
Chemicon |
CAS:149116-08-5 |
Benzamidine |
Sigma-Aldrich |
CAS:618-39-3 |
Traysol |
Bayer |
CAS:9087-70-1 |
|
Critical commercial assays |
|
QuantiChrom creatine assay kit |
BioAssay Systems |
Cat# DICT-500 |
Precision Xtra |
Abbott |
Cat# 9881465 |
Contour Next EZ |
Bayer |
Cat# 193725201 |
|
Experimental models: Organisms/strains |
|
"Ksp-Cre" Mice, B6.Cg-Tg(Cdh16-cre)91Igr/J |
Jackson Laboratory |
JAX:012237; MGI:4412080 |
Pkd1RC/RC Mice, p.Arg3277Cys |
University of Augusta, Augusta, Georgia |
|
Han Rat, Anks6PKD
|
|
RRID:RGD_11535000 |