Fig. 5.

The effects of stress exposure on gr transcript abundance in the adult zebrafish brain. (A) Zebrafish were exposed to air for 1 min and allowed to recover for up to 48 h (filled blue circles). (B) For a separate group of fish, the stressor was repeated 24 h after the first stressor and fish were allowed to recover for 15 min or 24 h (open blue circles). (C) Dominant (Dom) and subordinate (Sub) zebrafish after 24 h of social stress; group-housed (GH) fish are included as a control (purple squares). gr transcript abundance was normalized to the mean expression of two housekeeping genes (ef1α and rpl8). Data are shown as individual data points with a solid line and whiskers representing the mean±s.e.m., and statistical outliers as black triangles. Changes in gr transcript abundance in brain zone 2 (telencephalon and olfactory bulbs removed; A,B) and the whole brain (C) were determined using a one-way ANOVA followed by a Tukey's post hoc test (P<0.05). Within each panel, groups that do not share a common letter are significantly different from one another.