FIG. 6.

IE63 inhibits splicing in vitro and alters splicing complex formation. In vitro splicing assays were performed using adenovirus major late precursor mRNA with nuclear extracts from HSV-1 wt-infected (lanes 3), 27-lacZ-infected (lanes 2), or mock-infected (lanes 1) HeLa cells. The products of the splicing reaction were run on gels as follows. (A) A 10% polyacrylamide–8 M urea denaturing gel, to separate the RNA products formed. The pre-mRNA was ³²P labeled, and products were visualized by exposure to X-ray film overnight. (B) A nondenaturing agarose-polyacrylamide composite gel to separate the protein-RNA complexes formed. Complexes bound to labeled RNA were visualized by exposure to X-ray film overnight.