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. Author manuscript; available in PMC: 2024 Sep 23.
Published in final edited form as: Mol Cell. 2023 Dec 13;83(24):4586–4599.e5. doi: 10.1016/j.molcel.2023.11.007

Figure 3. Mechanism of SIR2 catalysis.

Figure 3

(A) NAD+ bins to the interface of the small domain and the large domain of SIR2.

(B) An expanded view of NAD+ coordination by key residues of SIR2.

(C) An expanded view of ADPR coordination by key residues of SIR2.

(D) Comparison of NAD+ hydrolase activity between HerA-SIR2 complex, HerA-SIR2H227A, HerA-SIR2D311A, HerA-SIR2H313A, and SIR2 apo dodecamer at 30 minutes. Mutations of residues impaired NAD+ hydrolysis. Data are mean ± SD from 3 replicates as indicated.

(E) Effects of ATP and ATPγS on NAD+ hydrolase activity of the HerA-SIR2 complex at different time points. Data are mean ± SD from 3 replicates as indicated.

(F) Effects of NTPs and dNTPs on NAD+ hydrolase activity of the HerA-SIR2 complex at 30 minutes. Data are mean ± SD from 3 replicates as indicated.

See also Figures S5.