TABLE 1.
Effects of preadsorbing with selected cell types on the FIV-neutralizing activity of FC vaccine sera and control infected and naive cat sera
| Serum no. | Titer of NAa with indicated cell type
|
||||||
|---|---|---|---|---|---|---|---|
| None | MBM | PBMC | PLB-d3 | PLB-d12 | CrFK | KB | |
| Vaccinated | |||||||
| 727 | <16 | 512 | <16 | <16 | 64 | 512 | 16 |
| 733 | <16 | 512 | <16 | <16 | 128 | 64 | <16 |
| 737 | <16 | 512 | <16 | <16 | 64 | 32 | <16 |
| 3607 | <16 | 512 | <16 | <16 | 128 | 512 | 16 |
| Infectedb | |||||||
| 753 | 256 | 512 | NDc | ND | ND | ND | ND |
| 792 | 512 | 512 | ND | ND | ND | ND | ND |
| 3573 | 256 | 512 | ND | ND | ND | ND | ND |
| 3588 | 512 | 512 | ND | ND | ND | ND | ND |
| 3603 | 512 | 512 | ND | ND | ND | ND | ND |
| Uninfected, unvaccinated (6 sera) | <16 | <16 | ND | ND | ND | ND | ND |
a
The titer is expressed as the reciprocal of the highest serum dilution that gave 50% inhibition of reverse transcriptase production by 10 50% tissue culture infectious doses of FIV-M2 mixed with the corresponding dilution of a pool of 10 normal cat sera and calculated by the Reed and Müench method (28). The experiment was repeated twice, with comparable results.
b
These cats were infected with plasma from FIV-M2-infected cats; sera were obtained 1 year later.
c
ND, not done.