Figure 2.

EB-SUN and EB1 localization and structure. (A) AlphaFold accurately predicts the homodimerization of EB1, consistent with previously reported crystal structures. (B) AlphaFold predicts that EB-SUN also forms a homodimer through interactions in the coiled-coil domain, with the N-terminus (The beginning of the coiled-coil domain) oriented to the left and the C-terminus to the right (dark blue for the most confidently predicted regions, via light blue and yellow to orange/red for the regions of very low confidence, as described in AlphaFold). (C) EB-SUN and EB1 colocalize at the growing tips of microtubules in S2 cells. Scale bar: 10 μm. (See Supplemental Movie S2).