Figure 1. Generation of a high-resolution scRNA-seq atlas of peripheral immune cells from healthy children and adults.

A. Overview of the Human Immune Health Atlas cohort (age range: 11–65 yrs; n=108) and final reference dataset. B. UMAP of immune cell subsets within the Atlas, highlighting major immune cell populations. C. Log₂ fold change of clinical metadata features of age and CMV infection status compared using Milo differential abundance testing. Bronze is higher in older adults and Teal is higher in young adults. Red is higher in CMV⁺ people and blue is higher in CMV⁻ people. D. Marker gene expression and cell counts of the 71 immune cell subsets in level 3 of the Atlas. More details about this Atlas can be found at https://apps.allenimmunology.org/aifi/resources/imm-health-atlas/.