Figure 2. Expression of NS2 complicates simple length-dependent replication kinetics.

a,b Barcoded 200, 400, 800, and 1600nt variants of each individual segment bearing equal sequence length derived from 5’ and 3’ ends and lacking canonical start codons were generated and co-transfected into HEK293T cells along with the minimal replication machinery with, or without, NS2. The relative frequency of each variant was measured by qPCR at 24h post-transfection, with 1600nt variants serving as a comparison. Asterisks indicate conditions significantly impacted by the expression of NS2, two-sample two-tailed t-test with a within-panel Benjamini-Hochberg corrected FDR<0.05. n=3 for (a), n=4 for (b), individual replicates and mean displayed.