Figure 1: Lipid composition of A. baumannii strain ATCC 17978 in excess and limiting phosphate concentrations.
A. Strains were grown in basal media 2 (BM2) with excess (1mM) or limiting (50 μM) phosphate. Cells were collected, lipids were extracted using the Bligh and Dyer method and separated using 2-dimensional thin-layer chromatography. Total lipids were stained with sulfuric acid (left). Aminolipids were stained using ninhydrin (right). Specific lipids are labelled: PE, phosphatidylethanolamine; PG, phosphatidylglycerol; CL, cardiolipin; LPE, lyso-PE; U1, unknown lipid 1; U2, unknown lipid 2; UPL1, unknown phospholipid 1. Red letters denote aminolipids that provide the focus of the study.