Figure 6: Aminolipid biosynthesis is conserved in Gram-negative ESKAPE pathogens.

2D thin-layer chromatography of lipids extracted from wild type K. pneumoniae strain KPNIH1, E. cloacae strain ATCC 13047, and P. aeruginosa strain PAO1 grown in excess (1 mM) or limiting (50 μM) phosphate conditions. Totals lipids were stained with sulfuric acid (left). Aminolipids were stained using ninhydrin (right). Specific lipids are labelled: PE, phosphatidylethanolamine; PG, phosphatidylglycerol; CL, cardiolipin; OL, ornithine lipid; U1, U2, U3, U4, and U5; unknown compounds 1, 2, 3, 4, and 5. B. Representative MS1 spectra from shotgun analysis of animolipids isolated from K. pneumoniae (n = 3) and E. cloacae (n = 3). The five most enriched lipids are bolded, including two ornithine lipids (OL), labelled in red.