Figure 1. Design and purification of DIS RNAs.

(A) The DIS-WT palindromic loop (yellow box) was mutated to either all C (red) or all G (blue) to ensure heterodimerization. A kinked-elongation element (blue) was added to DIS-Gk to distinguish it from DIS-C. (B) Preparative-scale size-exclusion chromatogram of the DIS RNAs. DIS-C was added in excess during complex formation to saturate DIS-Gk. (C) Native gel electrophoresis of the SEC-purified DIS RNAs. After SEC-purification, DIS-C:DIS-Gk complex remains monodisperse.