Figure 3. Contrast matching conditions and labeling scheme to probe individual DIS RNAs in complex.

(A) Calculated scattering length densities (ρ) of protiated RNA (yellow), ²H(42%) RNA (teal), and perdeuterated RNA (pink). Contrast between the chosen labeling schemes are denoted as Δρ. The contrast match points (Δρ=0) between the RNAs and appropriate H₂O:D₂O buffer are denoted by vertical dotted lines. (B) Labeling schemes used in our SANS experiments. The protiated RNA is matched out in 65% D₂O (left) and the ²H (42%) RNAs are matched out in 90% D₂O (right).