Extended Data Figure 1. Comparison of Cre expression patterns between transgenic lines targeted to the Pnmt locus.

a, Representative image from a Pnmt^Cre;Ai14 animal shows widespread ectopic expression of the Cre-dependent reporter tdTomato (red) beyond defined TH⁺ catecholaminergic cells (green) in the rostroventrolateral medulla (RVLM, dotted circles). n=3 animals. b, Representative images comparing expression of Cre-dependent AAV-GFP (green) injected into RVLM (dotted circles) in Pnmt^Cre (left) and Pnmt^2a-iCre (right) transgenic animals. Ectopic expression beyond RVLM catecholaminergic cells (TH, red) is greatly reduced in Pnmt^2a-iCre tissue (white arrowheads), suggesting improved restriction of Cre expression in these animals. Nuclei are counterstained with DAPI (blue). n=3 animals of each genotype.