Figure 1. Human primary cardiac cells are susceptible to infection by diverse arboviruses.

Human primary (A) cardiac fibroblasts, (B) aortic smooth muscle cells, (C) cardiac myocytes, and (D) cardiac microvascular endothelial cells were infected with MAYV, ZIKV, RVFV MP-12, or LACV at a MOI of 0.1. Supernatant was collected at 0, 6, 24, 48, and 72 hpi, and viral titers were quantified by plaque assay. Data points represent the mean of n = 2 independent trials with internal technical duplicates, with error bars representing the SEM. The limit of detection is indicated by the gray shaded area.