Figure 5. MAYV infection attenuates the type-I IFN response in cardiac endothelial cells.

(A-C) hCMECs were either pre-treated with Poly(I:C) or mock-transfected for 2 hours and then mock-infected with DMEM, or infected with CHIKV-IOL, CHIKV-181/25, MAYV, corresponding heat-inactivated controls (HI), or mock-infected (DMEM) at a MOI of 1 for 1 hour. Supernatants and cell monolayers were collected at 0 and 24 hpi . (A) Viral titers were quantified by plaque assay and (B) intracellular viral genomes were quantified by RT-qPCR. (C) ISG15 gene expression quantified relative to DMEM for each timepoint by RT-qPCR. Data represents the mean of n = 3 independent trials in technical duplicate with error bars representing the SEM and the limit of detection indicated by the gray shaded area. Statistical significance was found by (A - C) two-way ANOVA with multiple comparisons, with statistics in (A) representing comparisons to CHIKV-IOL at 24 hpi. P-values represent ****p<0.0001.