Figure 2. Monoclonal antibody detachment of HAstV previously bound to the surface of Caco-2 cells.

(A) HAstV1 or (B) HAstV2 was attached to cells on ice to prevent virus endocytosis. Subsequently, either ascitic fluid of (A) mAb 3B4 or 3H4, or (B) mAb 4B6 was added to cells and incubated for 1 h on ice. The remaining attached virus was determined by RT-qPCR. MAb 2D9 which is specific to serotype HAstV8 was used as a negative control. The assay was performed in biological sextuplicates and carried out in duplicate. The data are expressed as percentages of the virus that remained attached in the absence of antibodies and represent the mean ± SEM.