Figure 1.

Adult and neonatal mouse platelets have different immune molecule expression. A-D) P7 and adult platelet mRNA were isolated for RNA-seq. A) Heat Map and B) PCI plot of platelet mRNA expression demonstrated differential mRNA expression. C-D). Pathway analysis. Adult platelets were enriched for immune related transcripts compared to P7 platelets. E-G) Validation of RNA-seq. E) P7 and adult mouse plasma β2M were measured by ELISA. Adult plasma had more β2M compared to P7 plasma. F) Adult and P7 platelets were stimulated with thrombin and surface P-selectin determined by flow cytometry. Adult platelets had more P-selectin compared to P7 platelets. G) Human cord blood plasma had less β2M compared to adult blood plasma as determined by ELISA (mean ± SEM, Student t-test).