Figure 3:

Adult platelets induce a trafficking monocyte phenotype in vitro. Primary mouse BMM were co-incubated with adult or P7 platelets and 4 hrs later monocyte CD41, Ly6C, CCR2 and CCR5 expression were determined. A) Adult platelets formed significantly more PMAs compared to P7 platelets, but B) induced similar inflammatory monocyte phenotypes. C-D). Adult, but not P7, platelets induced a trafficking monocyte phenotype. Adult platelets increased C) CCR2 and D) CCR5 expression compared to P7 platelets (mean ± SEM, 1-way ANOVA with Bonferroni correction).