Table 2. Differential characteristics between the novel strain and the most related phylogenetic neighbours of genus Virgibacillus.
Strain: 1, C22-A2T; 2, V. halodenitrificans DSM 10037T; 3, V. salinus XH-22T; 4, V. indicus P2-C2T; 5, V. profoundi P3-H5T. +, Positive; −, negative; w, weakly positive; nd, not determined. All of the data were acquired from this experiment except where indicated otherwise.
| Characteristic | 1 | 2 | 3 | 4 | 5 |
| pH range (optimum) for growth | 7.0–10.0 (8.0) | 6.0–9.5 (7.0–7.5) | 6.0–10.0 (7.5) | 6.0–10.0 (7.5–8.0) | 6.5–9.0 (7.0) |
| Temperature range (optimum) for growth (°C) | 4–30 (20–25) | 4.0–42 (35) | 4.0–40 (35–37) | 4–40 (20–25) | 4–30 (15–25) |
| NaCl range (optimum) for growth (%, w/v) | 1.0–17 (3.0–7.0) | 0.5–20 (2.0–6.0) | 1.0–16 (2.0–4.0) | 0–16 (2.0–4.0) | 1.0–14 (4.0–6.0) |
| Utilization of substrates (Biolog GENIII): | |||||
| N-Acetyl-d-galactosamine | + | w | − | − | − |
| α-d-Glucose | − | + | − | + | w |
| d-Mannose | − | + | − | + | + |
| d-Frucose | − | + | w | w | + |
| d-Mannitol | + | + | − | − | + |
| l-Serine | + | + | − | + | + |
| Enzyme activity (API ZYM): | |||||
| Leucine arylamidase | + | + | w | + | − |
| N-Acetyl-β-glucosaminidase | − | + | − | − | − |
| Reactions (API 20E and 20NE): | |||||
| Urease | − | − | − | − | + |
| Hydrolysis of gelatin | + | + | − | + | + |
| Para-nitrophenyl-β-d-galactopyranosidase, PNPG | + | + | − | − | + |
| Orthon nitrophenyl-β-d-galactopyranosidase, ONPG | + | + | − | − | + |
| Tryptophane deaminase | − | − | − | − | − |