Figure 4.

Axonal growth rates within MCs and the control group

(A–C) Flat, RMC, and AMC for neuron culture.

(D–F) Images of the axon growth within the flat, RMC, and AMC groups after βIII-tubulin treatment. The axon extends from the left soma chamber to the axon chamber under the induction of shear stress. The red dotted line marks the outlet location of the MCs.

(G) Average time for the axons in AMCs and RMCs to reach the outlets.

(H) Average axonal growth rate within different MCs.

(I) Average axon length within different MCs. The data are presented as the mean ± standard deviation (n = 3). ∗p < 0.05. Scale bar: 120 μm.