Figure 5.

Axonal growth rates after extending beyond the outlet of the MCs

(A–C) Images of the axon growth outside the AMCs on days D₀+1, D₀+4, and D₀+7 after βIII tubulin treatment.

(D–F) Images of the axon growth outside the RMCs on days D₀+1, D₀+4, and D₀+7 after βIII tubulin treatment.

(G) Average axonal growth rate of the different MCs.

(H) Average axon length outside the AMCs and RMCs on days D₀+1, D₀+4, and D₀+7. Here, the average length of the axons represents the effective length, which is defined as the distance from the MC outlet to the axon terminal along the direction of the MC.

(I) Average number of axon terminals outside the AMCs and RMCs on days D₀+1, D₀+4, and D₀+7. Ten MCs were randomly selected for sampling, and the total number of axonal terminals within these MCs was quantified. Each distal tip of an axon is considered an axon terminal. Each axon has more than one terminal. The data are presented as the mean ± standard deviation (n = 3). ∗p < 0.05, ∗∗p < 0.01. Scale bar: 180 μm.