Figure 6.

Axon growth within the AMC chips and RMC chips on day 19

(A and B) Fluorescence images of axons within the microfluidic chips with AMCs and RMCs on day 19 after GFAP (red), βIII tubulin (green), and Hoechst 33258 (blue) treatment.

(C and D) Enlarged images of the axon terminal regions within the AMC chips and RMC chips.

(E) Average axon length within the AMC chips and RMC chips on day 19.

(F) Average number of axon terminals per 10 MCs within the AMC chips and RMC chips on day 19.

(G) Average number of cells that leaked per 10 MCs within AMC chips and RMC chips on days D₀+1, D₀+4, D₀+7, and 19. The data are presented as the mean ± standard deviation (n = 3). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. Scale bar: 130 μm.