Figure 7.

Axonal growth within AMC chips under different pressures

(A) AMC chips under pressure with ΔP_l, ΔP_m, and ΔP_h.

(B–D) Fluorescence images of axons within the AMC chips on day 19 under pressure with ΔP_l, ΔP_m, and ΔP_h after MAP-2 (green) and βIII tubulin (red) treatment.

(E) Average time for the axons within the AMCs under pressure with ΔP_l, ΔP_m, and ΔP_h to reach the outlets.

(F) Axonal growth rate outside the AMCs on days D₀+1, D₀+7, and 19 under pressure with ΔP_l, ΔP_m, and ΔP_h.

(G) Average axon length and average number of axon terminals per 10 MCs within the AMC chips on days D₀+1, D₀+7, and 19 under pressure with ΔP_l, ΔP_m, and ΔP_h. The data are presented as the mean ± standard deviation (n = 3). ∗p < 0.05, ∗∗p < 0.01. Scale bar: 230 μm.