REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse anti-βIII-tubulin	Abcam	Cat. #ab78078; RRID:AB_2256751
Rabbit Anti-MAP2	Abcam	Cat. #ab32454; RRID:AB_776174
Rabbit Anti-GFAP	Abcam	Cat. #ab68428; RRID:AB_1209224
Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)	Abcam	Cat. #ab150113; RRID:AB_2576208
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647)	Abcam	Cat. #ab150079; RRID:AB_2722623
Chemicals, peptides, and recombinant proteins
Neurobasal-A medium	Gibco	Cat. #10888022
B27	Gibco	Cat. #17504044
L-glutamine	Gibco	Cat. #A2916801
Glucose	Solarbio	Cat. #G8150
Penicillin/streptomycin	Solarbio	Cat. #P1400
Poly-L-lysine	Solarbio	Cat. #P8130
Collagen type I rat tail	Solarbio	Cat. #C8062
Hoechst 33258	Sigma-Aldrich	Cat. #94403
Experimental models: Organisms/strains
Sprague-Dawley rat	SiPeiFu Inc.	N/A
Software and algorithms
SigmaPlot	Systat Software Inc.	Grafiti LLC
Fiji	NIH Image	Fiji (imagej.net)
Other
Sylgard 184	Dow Corning	SYLGARD™ 184 Silicone Elastomer Kit | Dow Inc.