Figure 2.

Specificity of ndPCR assays for HERV-E TCR and linearity across a range of DNA inputs

(A) ndPCR performance of four different primer/probe sets to detect HERV-E TCR (TCR 1, 2, 3, and 4) using a purified population of HERV-E T cells from a male donor (93.1% transduction efficiency; VCN per transduced cell, 2.94) and PBMCs from a female donor as a negative control. All assays were tested in technical triplicates. Error bars represent mean ± SD. (B) Serial (half-log) dilutions of gDNA derived from purified HERV-E T cells were used to test the LOD of all four assays detecting the HERV-E TCR construct. All four assays exhibited linear and reproducible copy number quantifications of the vector insert and the RPPH reference gene across a range of DNA concentrations with high concordance. All assays were tested in technical triplicates.