Table 1.

Primer and probe sequences used in this study

Genes of interest	Primers and probes (5′-3′)
Ref_RPPH	F: AGTGAGTTCAATGGCTGAGG R: GGCGGAGGAGAGTAGTCT P:/5HEX/TTGGGTTAT/ZEN/GAGGTCCCCTGCG/31ABkFQ/
Ref_TERT	F: GGGTTGGCTGTGTTCCG R: GACATAAAAGAAAGACCTGAGCA P for duplex assays:/5HEX/AAGTTCCTG/ZEN/CACTGGCTGATGGT/31ABkFQ/ P for quadruplex assays:/5Cy5/AAGTTCCTG/ZEN/CACTGGCTGATGGT/31ABkFQ/
Ref_USP9Y	F: CAAGTATGACAGCCATCACTCA R: GTTGGAAGAGATGCTGAGACT P for duplex assays:/5HEX/CAGTAGGAG/ZEN/GGAACGACAGCCAG/3IABkFQ/ P for quadruplex assays:/5ROX/CAGTAGGAG/ZEN/GGAACGACAGCCAG/3IABkFQ/
TCR set 1	F: CTGGGTAACTTCAGTGTCTATG R: CCACGAACTTCTCTCTGTTAAA P:/56-FAM/CAAGCAGGA/ZEN/GACGTGGAGGAGAA/31ABkFQ/
TCR set 2	F: GCAAACTCAGCAAGCAAAG R: TGATGGCAATGGTCAAGAG P:/56-FAM/TGCTAACAT/ZEN/GCGGTGATGTCGAAG/31ABkFQ/
TCR set 3	F: GCAAACTCAGCAAGCAAAG R: GGATTTCGAATTCGGCTCA P:/56-FAM/TGCTAACAT/ZEN/GCGGTGATGTCGAAG/31ABkFQ/
TCR set 4	F: CCGTTGTTGTCAAGACTCATG R: TTCGAATTCGGCTCAGGC P:/56-FAM/CTGCTAACA/ZEN/TGCGGTGATGTCGAAG/31ABkFQ/