Table 1.

Characteristics of neoepitopes and their WT counterparts.

Mutant peptide	HLA-I	Sequencea	Affinity (nM) (WT/Mut)b	RMSD (Å)c	TM-scorec
KDM5C E656K	A0211	KMAACP[E→K]KL	40.09/251.67	0.13	0.94
HERC1 P3278S	A1101	ASNA[P→S]SAAK	21.93/21.11	0.70	0.43
SNX24 P132L	A0201	KLSHQ[P→L]VLL	85.09/25.78	0.18	0.91
PGM5 H469Y	A0201	AVGS[H→Y]VYSV	91.49/29.29	0.15	0.93
OXSM K109T	C0304	FVS[K→T]SDIKSM	929.87/565.01	0.52	0.67
TENM3 A2490V	A0211	GAQSWLWF[A→V]	109.77/5.66	1.23	0.41

^aPeptide sequence, with the mutation in the neoepitope in brackets.

^bPredicted affinity in nanomolar for peptide binding via Netmhcpan-4.1.

^cMeasured RMSD and TM-score between the neoepitope and WT counterpart when all common atoms of the peptides are superimposed.