Fig. 2. 6b is a potent and highly selective degrader of BRD4 protein.

A, B HCC1806 or HCC1937 cells were treated with 6b, 6d, 6e, 6f, and 6g at the indicated doses for 48 hours, protein expression was analyzed by WB and GAPDH was included as a loading control. C HCC1806 or HCC1937 cells were treated with 6b at the indicated doses for 48 hours for WB. D HCC1806 or HCC1937 cells were treated with 0.1 μM 6b for the indicated time for WB. E HCC1937 and HCC1806 cells were treated with 6b or DMSO (0.1 μM) for 12 h, then treated with cycloheximide (CHX, 50 μg/mL) for 1, 2, and 4 h. The cell lysates were collected for WB. The quantitative results are plotted below. GAPDH was used as the loading control. F HCC1937 and HCC1806 cells were treated with JQ1 (3 μM) or 6b (0-0.5-1 μM) for 48 h. The protein expression was measured by WB.