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. 2024 Aug 20;43(39):2914–2926. doi: 10.1038/s41388-024-03121-1

Fig. 3. 6b displays strong growth inhibition and cell cycle arrest in BLBC cell lines.

Fig. 3

A HCC1806 or HCC1937 cells were treated with 6b (0-0.2-0.4-0.8-1.6-3.2 μM) for 4 days for cell viability assay. B HCC1937 and HCC1806 cells were treated with 6b (0-0.05-0.1-0.2 μM) for 48 hours for Annexin V-PI apoptosis analysis. The quantitative results are plotted below. C HCC1937 and HCC1806 cells were treated with 6b (0-0.05-0.1-0.2 μM) for 48 hours and cell lysates were collected for WB. D 6b (0-0.05-0.1-0.2 μM) was used to treat HCC1937 and HCC1806 cells for 48 h, DNA synthesis was measured by the EdU assay, nuclei were stained with Hoechst 33342 (blue channel) and green channel represents EdU. The total numbers of cells and EdU-incorporated cells in each sample were counted under a fluorescent microscope, percentages of EdU-positive proliferating cells vs. total cells are shown. E Colony formatting unit assays of BLBC cells were performed on different formulations and incubated with the freshly prepared medium for another 9 day (n = 3). *p < 0.05, **p < 0.01. *** p < 0.001. t-test.