Skip to main content
. 2024 Aug 20;43(39):2914–2926. doi: 10.1038/s41388-024-03121-1

Fig. 7. 6b inhibits BLBC growth through KLF5 in part.

Fig. 7

A HCC1937 and HCC1806 cells were infected with expressing empty vector pCDH or KLF5 and were analyzed for expression of the transduced proteins by WB using antibodies against KLF5. GAPDH was used to show equal protein loading. Relative growth is shown below. B HCC1937 and HCC1806 cells were infected with shRNA expressing an empty vector shControl or shKLF5-3’UTR/#5/#7 were analyzed for expression. GAPDH was used to show equal protein loading. Relative growth is shown below. C HCC1937 and HCC1806 cells were treated with 0-0.25-0.5-1 μM of 6b for 48 h and protein expression was analyzed by WB using indicated antibodies. D HCC1937 and HCC1806 cells were treated with 0.1-1 μM of 6b with FZU004, EPI, or PTX for 48 h and protein expression was analyzed by WB using indicated antibodies. Cell viability shown below is measured by SRB assays, the quantitative data are compared to negative control. E Cells stably over-expressing or knockdown KLF5 relative growth was detected by SRB assay. F HCC1806 cells were treated with 6b plus FZU004 or EPI for 48 h, stained with Annexin V/7AAD, and analyzed by flow cytometry, the quantitative data and percentages of Annexin V-positive cells are shown. *p < 0.05, **p < 0.01. ***p < 0.001. t-test.