FIG. 3.

Proteins recognized by MAb 5C6 under nonreducing conditions on a Western blot. Cell extracts (100 μg of protein each) from HeLa, Vero, B95a, and BJAB cells, PHA-activated human PBMC and PBL, and selected transduced (CHO-SLAM) and parental CHO cells (lanes 1 to 8) were separated by SDS-PAGE on an 8% gel and blotted (semidry) on a polyrinylidene difluoride membrane. The blot was incubated with MAb 5C6 and peroxidase-conjugated secondary antibody, and bands were visualized using an enhanced chemiluminescence system (Amersham).