FIG. 4.

MV binding to and susceptibility of CHO-SLAM and CHO-CD46 cells. FACS-sorted CHO-SLAM cells express high amounts of SLAM at their cell surface (a). CHO-SLAM, CHO-CD46, and parental CHO cells were incubated with increasing amounts (MOI = 0.5, 1, and 2) of Edm (b) and WTFb (c), and bound virus was detected by flow cytometry. CHO-SLAM (d and e) and CHO (f and g) cells were incubated with MV Edm (d and f) or WTFb (e and g) for 48 h (MOI = 1), and syncytium formation observed in the microscope. Syncytia were present only in SLAM-transduced cells. The bar in panel e represents 50 μm.