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. 2024 Sep 23;7:1187. doi: 10.1038/s42003-024-06889-w

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Analysis of 20–30 s time-lapse segments of CaMyo51B-GFP expressing cells during a starting, b stopping, and c reversing. (Left panels) Montages showing the position of cells at 5 s intervals (GFP in green, chloroplast autofluorescence in magenta, scale bars: 5 µm). (Middle panels) Cell velocity as a function of time, generated from chloroplast tracking data. (Right panels) Registered kymographs generated from GFP-channel data (black) showing movement of myosins relative to the cell. The small offsets between starting/stopping/reversing of cell and myosin activity (dotted horizontal lines between middle and right panels) likely indicate mechanical compliance in the motility machinery. Gray ellipses and white slits above kymographs approximate the positions of the cell body and raphe openings, respectively. (Scale bars: horizontal = 5 µm, vertical = 5 s).