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. 2024 Sep 23;7:1187. doi: 10.1038/s42003-024-06889-w

Fig. 6. Multi-directional movements of CaMyo51C-GFP and CaMyo51D-GFP.

Fig. 6

Analysis of 25-60 s time-lapse segments of CaMyo51C-GFP and CaMyo51D-GFP expressing cells showing a CaMyo51C-GFP movement from apices to the cell center (red arrows) in a cell exhibiting repeated changes in gliding velocity, b CaMyo51D-GFP movement from apices to the cell center (red arrows) in a cell exhibiting slow unidirectional gliding, and c CaMyo51D-GFP movement from the center to the cell apices (yellow arrows) in a mostly stationary cell. (Left panels) Montages showing the position of cells at 5 s intervals (GFP in green, chloroplast autofluorescence in magenta, scale bars: 5 µm). (Middle panels) Cell velocity as function of time, generated from chloroplast tracking data. (Right panels) Registered kymographs generated from GFP-channel data (black) showing movement of myosins relative to the cell. Gray ellipses and white slits above kymographs approximate the positions of the cell body and raphe openings, respectively. (Scale bars: horizontal = 5 µm, vertical = 5 s).