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Quantitative immunofluorescence detects changes in the immune microenvironment in patients with advanced EGFR-mutant NSCLC treated with afatinib and pembrolizumab. (A) Patient with 20% tumor shrinkage and PFS more than 6 months. Increase in CD3+ T-cells and decrease in tumor proliferation (Ki67) noted. (B) Patient with progression at first interval imaging. No change in CD3+ T-cells or tumor proliferation (Ki67). PFS, progression-free survival.
