Figure 5.

Inhibition of preautophagosomal maturation by UPEC PldA protects against autophagy in host cells. A, Colocalization of NDP52 with the WT strain expressing GFP, ΔpldA expressing mCherry, or both strains at a 1:1 ratio in 5637 cells at 4 hpi. The arrow indicates NDP52+ bacteria, and the square frame indicates NDP52 granules. Scale bar, 5 μm. B, Quantification of the intracellular bacteria that colocalized with NDP52 relative to the total number of cells infected with either ΔpldA-mCherry alone or WT-GFP and ΔpldA-mCherry (coinfection). n = 3 slides. Data are presented as the mean ± SD. P values were determined using Student t test. Significance is indicated by the P value. Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole; GFP, green fluorescent protein; hpi, hours postinfection; ns, not significant; UPEC, uropathogenic Escherichia coli; WT, wild type.