Figure 2.

Direct binding measurements of fluorescein-labeled truncated peptides to PKA catalytic subunit determined by fluorescence polarization. StIP-3 and StIP-4 demonstrate low nanomolar binding to PKA with truncated sequences compared to the parent peptide sequence, StIP. * = location where (S)-2-(4-pentenyl)alanine is incorporated into the peptide sequence to form the hydrocarbon staple. Mean values and standard deviation of three independent measurements are given. n.d. = not determined. Both StIP-T3 and StIP-T4 were found to have low-nanomolar affinities.