Figure 5.

CEN cells require costimulation for proliferation and can weakly suppress CD4⁺CD45RO^–CD25^– cells. (A) Separated CEN (black bars) or naive CD25^– cells (white bars) from IL-2–treated patients were cultured in the presence of media, anti-CD3, or PHA for 96 hours. Media (background) proliferation was subtracted. Median values from 9 independent experiments using different donors are shown. (B) Separated CD4⁺CD25^– cells were cultured at fixed numbers either alone (white bars) or in the presence of CD4⁺CD25⁺ cells at ratios of 1:2 (black bars) or 1:1 (gray bars). Median values from 7 independent experiments using different donors are shown.