Figure 7.

CEN cells from IL-2–treated patients exert weak suppression compared with that of Tregs. (A) Proliferative responses of separated subsets to anti-CD3 as measured by tritiated thymidine incorporation. Median values from 4 independent experiments using different donors are shown. (B) CD4⁺CD45RO^–CD25^– cells were stained with CFSE and stimulated for 96 hours with anti-CD3 (1 μg/ml) with or without the addition of either CD45RO^–CD25⁺ or CD45RO⁺CD25^+high subsets from the same patient at different ratios. Gating was done on CD4⁺CFSE⁺ cells. One of 8 experiments from independent donors is shown.