Figure 3.

The gene expression levels of CD8, IFN-γ, and IL-10 in the tumor microenvironment were altered by local rV-B7.1 vaccination. RNA was amplified from FNAs obtained before and after vaccination. (A) Logarithmic changes of target genes corrected by PKG-1 endogenous control values are shown for 5 index lesions. Postvaccination gene expression of CD8, IFN-γ, and IL-10 in index lesions from patients 3, 4, 5, 7 and 9 classified as SD, PR, or PD are shown. ND, not determined. (B) Postvaccination gene expression levels of CD8, IFN-γ, and IL-10 are shown for patient 4, classified as PR, throughout the administration of 3 vaccine cycles (9 injections of rV-B7.1). The specific injection number within each cycle is indicated. Post, postvaccination; pre, prevaccination. (C) Measurements of the index lesion area (cm²) of patient 4 corresponding to the gene transcript levels in B are shown.