Fig. 2.

Effect of nuclear displacement on contractile ring assembly. (a) Asynchronous wild-type cells were centrifuged and assayed for positions of the spindle, nucleus, and rlc1-GFP by using time-lapse microscopy. Percentages of cells forming contractile rings or ring fragments (as visualized by rlc1-GFP) at the specified positions are listed. (b) cdc25-22 cells were synchronized in mitosis by temperature shifts, centrifuged at specified cell-cycle points, and then imaged. (c) cdc25-22 cells were synchronized and centrifuged twice (see Materials and Methods).