Fig. 4.

The nucleus positions mid1p at the cortex. (a–c) Time-lapse images (Left) of cells expressing mid1-GFP. Kymographs (Center and Right) depict mid1-GFP behavior in boxed regions located at the cortex (c) and the nuclear envelope (n). (a) Interphase mid1-GFP cells (FC958) without centrifugation. (b and c) Two representative examples of interphase mid1-GFP cells in which the nucleus is recentering, after centrifugation. An arrow indicates large nuclear envelope deformation associated with nuclear movement. Asterisks mark representative mid1-GFP cortical dots that move with the nucleus. (Scale bars, 3 μm; kymographs, ×1.5.)