TABLE 2.
Immunization of hamsters with rB/HPIV3 expressing the RSV G or F ORF as an additional gene induces a serum antibody response against the RSV G or F protein, respectively
| Immunizing virusa | No. of animals/group | Serum immunoglobulin G ELISA titer (mean reciprocal log, ± SE) against RSV proteinb:
|
|||||
|---|---|---|---|---|---|---|---|
| G protein
|
F protein
|
||||||
| Prec | Day 26 | Log2 fold increase | Prec | Day 26 | Log2 fold increase | ||
| rB-HPIV3-G1 | 12 | 6.0 ± 0.4 | 12.5 ± 0.5 | 6.5 | 6.7 ± 0.5 | 7.5 ± 0.5 | 0.8 |
| rB/HPIV3-F1 | 12 | 6.3 ± 0.3 | 7.2 ± 0.3 | 0.9 | 6.8 ± 0.3 | 16.2 ± 0.5 | 9.4 |
| rB/HPIV3-G1 and rB/HPIV3-F1 | 12 | 6.5 ± 0.6 | 12.0 ± 0.9 | 5.5 | 7.3 ± 0.5 | 14.7 ± 0.4 | 7.4 |
| rB/HPIV3 | 12 | 6.5 ± 0.4 | 8.0 ± 0.4 | 1.5 | 7.3 ± 0.7 | 8.3 ± 0.8 | 1.0 |
| RSV | 12 | 6.8 ± 0.3 | 10.8 ± 0.4 | 4.0 | 7.5 ± 0.5 | 15.7 ± 0.4 | 8.2 |
a
Hamsters were inoculated intranasally with 106 TCID50 of virus in a 0.1-ml inoculum.
b
Serum samples were taken 3 days prior to inoculation (Pre) and 26 days postinoculation and analyzed by glycoprotein-specific ELISA for antibodies against RSV G or F protein, as indicated.
c
Titers in the “Pre” serum specimen represent nonspecific background levels of antibody in this sensitive ELISA.