TABLE 3.
Immunization of hamsters with rB/HPIV3 viruses expressing the RSV G or F ORF induces neutralizing serum antibodies against RSV as well as hemagglutination-inhibiting antibodies against HPIV3
| Immunizing virusa | No. of animals/group | Neutralizing serum antibody response to RSVb (mean reciprocal log2 ± SEd)
|
Serum hemagglutination-inhibiting antibody response to HPIV3c (mean reciprocal log2 ± SEd)
|
||
|---|---|---|---|---|---|
| Pre | Day 26 | Pre | Day 26 | ||
| rB/HPIV3-G1 | 12 | ≤3.3 | 10.0 ± 0.3 (A) | ≤2 | 10.0 ± 0.5 (A) |
| rB/HPIV3-F1 | 12 | ≤3.3 | 9.3 ± 0.5 (A) | ≤2 | 8.8 ± 0.1 (A) |
| rB/HPIV3-G1 and rB/HPIV3-F1 | 12 | ≤3.3 | 10.8 ± 0.4 (A) | ≤2 | 8.8 ± 0.3 (A) |
| rB/HPIV3 | 12 | ≤3.3 | ≤3.3 (B) | ≤2 | 9.5 ± 0.8 (A) |
| RSV | 12 | ≤3.3 | 8.1 ± 1.2 (A) | ≤2 | ≤2 (B) |
a
Hamsters were inoculated intranasally with 106 TCID50 of the indicated PIV3 or 106 PFU of RSV in a 0.1-ml inoculum.
b
Serum samples were taken on day 26 postinoculation, and antibody titers were determined by 60% plaque reduction neutralization test.
c
Serum samples were taken on day 26 postinoculation, and antibody titers were determined by HAI test.
d
Mean virus titers were assigned to similar groups (A and B) by the Tukey-Kramer test. Within each column, mean titers with different letters are statistically different (P < 0.05).