| coculturing |
phenylpropene |
tripartite coculture of E. coli to spread metabolic burden across organisms |
(76) |
| coculturing |
(S)-norcoclaurine |
Scheffersomyces
stipitis produces shikimate, which S. cerevisiae converts to (S)-norcoclaurine |
(72) |
| strain engineering |
armeniaspirols |
knockout of competing polyketide
cluster in Streptomyces sp. A793 and
introduction of heterologous fatty acid synthase to ease bottleneck
of precursors from primary metabolism |
(77) |
| strain
engineering |
citramalate |
transposon-guided copy number
engineering of Issatchenkia orientalis, which can grow at low pH |
(78) |
| strain
engineering |
pamamycin |
pamamycin-resistant Streptomyces albus J1074 underwent transcriptional
engineering by screening of a promoter library |
(79) |
| cell-free
synthesis |
salivaricin B |
one-pot reaction of engineered E. coli extract with chaperone proteins, precursor
peptides, and tailoring enzymes |
(80) |
