REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti – Ki67	Abcam	Cat# Ab16667; RRID: AB_302459
Anti – CD45	Thermo Fisher	Cat# Pa5-96061; RRID: AB_2807863
Donkey Anti-Rabbit Cy5	Jackson Immuno Research	Cat# 711-175-152; RRID: AB_2340607
DAB Secondary Kit	Vector	Cat # MP-7801-15
Chemicals, peptides, and recombinant proteins
BAPN	Sigma	A3134-SG
PBS	Thermo Fisher	BP399-20
PFA	Thermo Fisher	J19943-K2
EDTA	Fisher	5316–212
Sucrose	Thermo Fisher	A15583.0C
OCT	Thermo Fisher	4585
Mounting Medium	Electron Microscopy Sciences	16900
DAPI-containing Mounting Medium	Invitrogen	P36931
Picrosirius Red	Thermo Fisher	00059437
Alcian blue	Poly Scientific	S111A
Horse Serum	Vector	S-2012
RNAse Away	MBP	7002
Trizol	Invitrogen	15596018
Chloroform	Thermo Fisher	BP1145
Ethanol	Thermo Fisher	BP2818-500
Critical commercial assays
ClickIt Edu Kit	Thermo Fisher	C10337
RNeasy Micro Kit	Qiagen	74004
Deposited data
Bulk sequencing and analysis of mouse annulus fibrosus tissue	This paper	GEO: GSE275620
Experimental models: Organisms/strains
C57bl/6J WT	The Jackson Laboratory	000664
C57bl/6j ScxGFP	Bred In house	Originated from Dr. Ronen Schweitzer
Software and algorithms
ImageJ	National Inst. Of Health	https://imagej.nih.gov/nih-image/index.html
GraphPad Prism	Graphpad	https://www.graphpad.com/
Other
26 G needle	BD	305111
27 G needle	Exel Int	26426
30 G needle	BD	305128
31 G needle	BD	328446
36 G needle	WPI	NF36BV
8-0 Prolene Sutures	Ethicon	873OH
Needle Stopper for 30 G	McMaster Car	20H304
Needle Stopper for 27/26 G	McMaster Car	21H304
AFM Tips	Novascan	PT.PS
Phasemaker Tubes	Invitrogen	A33248
Blue India Ink	Thermo Fisher	3120124
Histological Slides	ASI	SM2575