FIG. 1.
Binding of respiroviruses to mixtures of gangliosides in virus overlay assays. Total gangliosides (each 5 nmol as sialic acid) and specific kinds of gangliosides (1 nmol) were spotted on silica gel plastic plates and subjected to chromatography with solvent system 1. (A) Gangliosides were detected with resorcinol-hydrochloric acid reagent. (B to D) Ganglioside binding by SV (B), hPIV-1 (C), and hPIV-3 (D) was detected by using virus overlay assays and anti-HN MAbs specific for each virus. (E) A chromatogram was incubated without virus and later incubated with a mixture of anti-HN MAbs. Lanes 1, GM3, GM1a, and GD1a; lanes 2, total gangliosides from bovine brain; lanes 3, total gangliosides from human placenta; lanes 4, total gangliosides from human meconium; lanes 5, NeuAcα2–3PG and NeuAcα2–3I.