FIG. 5.
Binding of respiroviruses to blood group I-type gangliosides containing different terminal molecular species of sialic acid in virus overlay assays. Specific types of gangliosides (1 nmol) were spotted on silica gel plastic plates and subjected to chromatography with solvent system 2. (A) Gangliosides were detected with resorcinol-hydrochloric acid reagent. (B to D) Virus overlay assays with SV (B), hPIV-1 (C), and hPIV-3 (D) were done as described in the legend to Fig. 1 and in Materials and Methods. (E) A chromatogram that was incubated without virus and with a mixture of anti-HN MAbs served as a negative control. Lanes 1, NeuGcα2–3I; lanes 2, NeuAcα2–3I; lanes 3, NeuAcα2–6I; lanes 4, GM1a, GD1a, and GQ1b.